Accurately calculate your dosage volume for lyophilized peptides
Wash hands thoroughly and don sterile gloves and eye protection. Ensure your workspace is impeccably clean. You will need: lyophilized peptide vial, sterile syringe, bacteriostatic water, and sterile swabs.
Before reconstituting, bring both the peptide powder and bacteriostatic water to room temperature. Using cold solutions may interfere with proper dissolution.
Remove the central metal cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Repeat this step for the bacteriostatic water vial. Allow to air dry for 30 seconds.
Using a sterile syringe, withdraw the required volume of bacteriostatic water. Slowly inject it into the peptide vial, tilting at a 45° angle so the liquid runs down the interior wall — this minimizes bubble formation and ensures smooth dissolution.
Gently swirl the vial in a circular motion until the powder is fully dissolved. Do not shake vigorously, as this can degrade peptide integrity.
Store reconstituted solution at +4°C (refrigerator) for short-term use — stable for 3+ weeks. For long-term storage, freeze aliquots at −20°C for 3–4 months. Avoid repeated freeze-thaw cycles to preserve potency.